| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.132032 |
| Chromosome: | chromosome 12 |
| Location: | 5896436 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g534000 | (1 of 58) PTHR12393//PTHR12393:SF6 - SPHINGOMYELIN PHOSPHODIESTERASE RELATED // SPHINGOMYELIN PHOSPHODIESTERASE 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTAAGGGCGGGTTGGGGTGTTTGTGCAT |
| Internal bar code: | CTTATGTCGATGGCGTGCGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1004 |
| LEAP-Seq percent confirming: | 99.8223 |
| LEAP-Seq n confirming: | 15728 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAATGAACTGGTTCGCCT |
| Suggested primer 2: | ACGCTGCAGTGTTGGTAGTG |