| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.132044 |
| Chromosome: | chromosome 3 |
| Location: | 5019304 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g180600 | FPN3 | (1 of 3) K01081 - 5'-nucleotidase (E3.1.3.5); Flagellar Associated 5'-nucleotidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGTGCGCGTGGAGCTGCCGGGCGGCGAC |
| Internal bar code: | GGGCCGATGGCTGATACGTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 756 |
| LEAP-Seq percent confirming: | 99.7521 |
| LEAP-Seq n confirming: | 4426 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGGTCAAATTTTGCGTC |
| Suggested primer 2: | TCTCAAGGAACGTGCAGATG |