| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.132057 |
| Chromosome: | chromosome 6 |
| Location: | 5733048 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g287000 | (1 of 1) PTHR31495//PTHR31495:SF0 - FAMILY NOT NAMED // PEROXYGENASE 1-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTGTGGAGTTGCGTGTCTTGATTGCCCG |
| Internal bar code: | TCCGTCTCGCTACTAGCATGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1292 |
| LEAP-Seq percent confirming: | 99.5219 |
| LEAP-Seq n confirming: | 14780 |
| LEAP-Seq n nonconfirming: | 71 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTTCAAGAGTTTGGCGAC |
| Suggested primer 2: | AGCATGCAACGTTCTCCTCT |