| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.132069 |
| Chromosome: | chromosome 10 |
| Location: | 2919252 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g440250 | PGM19 | Putative phosphoglycerate mutase; (1 of 1) 3.1.3.80 - 2,3-bisphosphoglycerate 3-phosphatase / 2,3-BPG 3-phosphatase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCTCGGCGCGGGCCCCGACCCTTGCCCC |
| Internal bar code: | AAGTACCAAGAATGGCGCGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 800 |
| LEAP-Seq percent confirming: | 99.8178 |
| LEAP-Seq n confirming: | 3288 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGCTTGGCAAAATGACTG |
| Suggested primer 2: | CGAAGAACTGAGGCAAGTCC |