| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.132071 |
| Chromosome: | chromosome 1 |
| Location: | 2797758 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g016570 | (1 of 1) IPR000104//IPR000719//IPR001245//IPR002290//IPR011009//IPR016024//IPR020635 - Antifreeze protein, type I // Protein kinase domain // Serine-threonine/tyrosine-protein kinase catalytic domain // Serine/threonine/dual specificity protein kinase, catalytic domain // Protein kinase-like domain // Armadillo-type fold // Tyrosine-protein kinase, catalytic domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGGCCCAAACTAAACTTGGCCCAAACTA |
| Internal bar code: | GCCTAGAATTCTGGGCATCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 392 |
| LEAP-Seq percent confirming: | 99.6336 |
| LEAP-Seq n confirming: | 2447 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCGTACTGTCCGACCTGT |
| Suggested primer 2: | ATGCATGTAACCCCTTCCAG |