Insertion junction: LMJ.RY0402.132079_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.132079
Chromosome:chromosome 11
Location:491732
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467597 (1 of 1) IPR000104//IPR003439//IPR003593//IPR013525//IPR013581//IPR027417 - Antifreeze protein, type I // ABC transporter-like // AAA+ ATPase domain // ABC-2 type transporter // Plant PDR ABC transporter associated // P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGGTAGGTGGTGTCGGTTTGGGAGGGGG
Internal bar code:CCTCGATTACTCTGCTTCGAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:214
LEAP-Seq percent confirming:98.8687
LEAP-Seq n confirming:8040
LEAP-Seq n nonconfirming:92
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:ACCCGTACATCTGCTCAACC
Suggested primer 2:AGCCCAAGTAGGGAGGGTAA