| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.132086 |
| Chromosome: | chromosome 6 |
| Location: | 1253432 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g258000 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACCCGCCTCTCGTCTTCTACACTACACC |
| Internal bar code: | CGTAATTGCACAAGAATTTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 915 |
| LEAP-Seq percent confirming: | 93.948 |
| LEAP-Seq n confirming: | 13552 |
| LEAP-Seq n nonconfirming: | 873 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGAATGTGTTGCATGCGT |
| Suggested primer 2: | TGTGCAATGCCTTGACTCTC |