| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.132100 |
| Chromosome: | chromosome 6 |
| Location: | 3693710 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278142 | (1 of 1) K14441 - ribosomal protein S12 methylthiotransferase [EC:2.8.4.4] (rimO) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGACGTTCAAGTGCGTGGTTTTCGGGGCG |
| Internal bar code: | ATGGCCGTGTATAGGATCGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 700 |
| LEAP-Seq percent confirming: | 99.6169 |
| LEAP-Seq n confirming: | 9361 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTCGGCAGGGTAGAAGAG |
| Suggested primer 2: | TATGCTTGACAACGGAGTGG |