| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.132101 |
| Chromosome: | chromosome_16 |
| Location: | 1435243 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g652400 | FAL18 | Similar to Flagellar Associated Protein FAP183 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTGCGCGGCTGCCGTATCACCCGCTGGCTC |
| Internal bar code: | CAGGTGTGGGAGGATGTACCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 231 |
| LEAP-Seq percent confirming: | 99.6 |
| LEAP-Seq n confirming: | 249 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACCAATACCTCTGCCCCC |
| Suggested primer 2: | ATGTATTTGCTCCCCGTGTC |