Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.132129 |
Chromosome: | chromosome_17 |
Location: | 3217755 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g722350 | RABL2A | Small Rab-related GTPase | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTTGTATTGTGAGGGTGGAGGAGTGAGTGG |
Internal bar code: | ACCTTGCGCACGAGATCAAATC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 946 |
LEAP-Seq percent confirming: | 93.0599 |
LEAP-Seq n confirming: | 3245 |
LEAP-Seq n nonconfirming: | 242 |
LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TACCCTGACAGGTGACCTCC |
Suggested primer 2: | TGTGCATCATGACGGGTACT |