| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.132129 |
| Chromosome: | chromosome_17 |
| Location: | 3217765 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g722350 | RABL2A | Small Rab-related GTPase | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCTGATTGTCTTCCCACGTACAGCGGCAAC |
| Internal bar code: | GGGGTCTTGTCTTGTACTCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1020 |
| LEAP-Seq percent confirming: | 99.6586 |
| LEAP-Seq n confirming: | 21310 |
| LEAP-Seq n nonconfirming: | 73 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCCTGACAGGTGACCTCC |
| Suggested primer 2: | TGTGCATCATGACGGGTACT |