| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.132130 |
| Chromosome: | chromosome 12 |
| Location: | 8256095 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g550300 | (1 of 1) PTHR24361//PTHR24361:SF276 - MITOGEN-ACTIVATED KINASE KINASE KINASE // PROTEIN KINASE | CDS|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAACAGCGCCGCGGGCGGCGCCTTCGTCG |
| Internal bar code: | GAGCGGTGTCTGAGCGGCGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 582 |
| LEAP-Seq percent confirming: | 97.1429 |
| LEAP-Seq n confirming: | 612 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTGACGCAACTACCACAA |
| Suggested primer 2: | GGTAGATGGAGGGAACCAGG |