Insertion junction: LMJ.RY0402.132133_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.132133
Chromosome:chromosome 9
Location:24980
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g386119 (1 of 2) PF13383 - Methyltransferase domain (Methyltransf_22) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATCTACATGCTTGCAGGCTTTGAGTGGGG
Internal bar code:CCGGGAGACAATTCCGGTGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:716
LEAP-Seq percent confirming:81.7104
LEAP-Seq n confirming:9994
LEAP-Seq n nonconfirming:2237
LEAP-Seq n unique pos:100

Suggested primers for confirmation by PCR

Suggested primer 1:GACTTGATTCCGCGTCTCTC
Suggested primer 2:ATTAGTCGCCAGACCCACAC