| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.132160 |
| Chromosome: | chromosome 9 |
| Location: | 1077242 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400500 | (1 of 3) PF14494 - Domain of unknown function (DUF4436) (DUF4436) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATCGCCCCCGCCACCTATTTGGTTTAGT |
| Internal bar code: | TGTGTGGCGCTCCAAGGCGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 363 |
| LEAP-Seq percent confirming: | 90.3614 |
| LEAP-Seq n confirming: | 75 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCAACCTTACCATGTTCG |
| Suggested primer 2: | AGTCGGGCAGTTTACATTGG |