Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.132166 |
Chromosome: | chromosome 7 |
Location: | 2874875 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g332300 | GWD2,1 | Alpha-glucan water dikinase 2; (1 of 3) 2.7.9.4 - Alpha-glucan, water dikinase / Starch-related R1 protein | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAAGTGCCGGCGGGGTGCGCGCACATGT |
Internal bar code: | TCTTGCGGCGGCCTATTACTGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 324 |
LEAP-Seq percent confirming: | 99.7383 |
LEAP-Seq n confirming: | 2287 |
LEAP-Seq n nonconfirming: | 6 |
LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATGTAACCCCCTCCCATCTC |
Suggested primer 2: | TGTGTGAGTGTGTGGGTGTG |