Insertion junction: LMJ.RY0402.132170_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.132170
Chromosome:chromosome_6
Location:5442996
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre06.g284300 antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):GCATAATCATAAAAGCAACAATGCGTGTTG
Internal bar code:GGTGAGTTTGTGCACGCCAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:931
LEAP-Seq percent confirming:91.7354
LEAP-Seq n confirming:8114
LEAP-Seq n nonconfirming:731
LEAP-Seq n unique pos:71

Suggested primers for confirmation by PCR

Suggested primer 1:CGCACTTCAGGCATCTGTTA
Suggested primer 2:GAGACTAACCAAGCCGCAAG