Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.132182 |
Chromosome: | chromosome_17 |
Location: | 3091724 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g721000 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CACGAACACCGTGCCACAACTTATCTCGAA |
Internal bar code: | ACAGAAGAATAGCATAAAGGCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1313 |
LEAP-Seq percent confirming: | 99.6229 |
LEAP-Seq n confirming: | 2642 |
LEAP-Seq n nonconfirming: | 10 |
LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGTGTGGTGTGGAGTACACG |
Suggested primer 2: | TGGAAGTGACTGCAGACTGG |