Insertion junction: LMJ.RY0402.132204_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.132204
Chromosome:chromosome 11
Location:1074684
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467675 (1 of 6) PF12796//PF13920 - Ankyrin repeats (3 copies) (Ank_2) // Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTACCCGGACCCAGATTTTACGTTTTGCC
Internal bar code:ACGGGTAACGCAGACGCAGACG

Confirmation - LEAP-Seq

LEAP-Seq distance:836
LEAP-Seq percent confirming:94.5065
LEAP-Seq n confirming:5161
LEAP-Seq n nonconfirming:300
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:CACCCCTACTTAGCACGCTC
Suggested primer 2:GTTTGTTTGTGTGTGGGTGC