Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.132227 |
Chromosome: | chromosome_10 |
Location: | 1757152 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g430700 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATGTGCCAGTTGAGGCCGTACGGTTGCATG |
Internal bar code: | CCCGTCACCTTATAGTACTATT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 894 |
LEAP-Seq percent confirming: | 99.6439 |
LEAP-Seq n confirming: | 5037 |
LEAP-Seq n nonconfirming: | 18 |
LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATACGGCACCTTACGACCTG |
Suggested primer 2: | TTGAAAGTTCGTTCGGCTCT |