| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.132251 |
| Chromosome: | chromosome 12 |
| Location: | 5843299 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g533700 | CYA12 | Solute-binding protein-like adenylate cyclase; (1 of 3) IPR000104//IPR001054//IPR006059//IPR029787 - Antifreeze protein, type I // Adenylyl cyclase class-3/4/guanylyl cyclase // Solute-binding family 1 // Nucleotide cyclase | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTGTCCGTCACCGTCCCCGCGCAGGTGC |
| Internal bar code: | TTGGACGTTTTGGACGGGCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1000 |
| LEAP-Seq percent confirming: | 99.6211 |
| LEAP-Seq n confirming: | 9464 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGGAATGAGGCGTGAGAA |
| Suggested primer 2: | ATGTGTAATCCCGGAGCAAG |