Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.132267 |
Chromosome: | chromosome_15 |
Location: | 783258 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre15.g640000 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATGCATGTATGCGCAGGGCGCGGCGTCGCC |
Internal bar code: | GTTTCTCGGCGAACTTCGTCTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 632 |
LEAP-Seq percent confirming: | 99.0421 |
LEAP-Seq n confirming: | 6617 |
LEAP-Seq n nonconfirming: | 64 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TATGCGATTGTCAAAGCTGC |
Suggested primer 2: | TATTTACAACCCACCCCGAA |