| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.132267 |
| Chromosome: | chromosome 15 |
| Location: | 783258 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g640000 | FAP345 | Flagellar Associated Protein 345 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCATGTATGCGCAGGGCGCGGCGTCGCC |
| Internal bar code: | GTTTCTCGGCGAACTTCGTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 632 |
| LEAP-Seq percent confirming: | 99.0421 |
| LEAP-Seq n confirming: | 6617 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGCGATTGTCAAAGCTGC |
| Suggested primer 2: | TATTTACAACCCACCCCGAA |