| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.132287 |
| Chromosome: | chromosome 6 |
| Location: | 1172714 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g257300 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTGTAGGTACGCCAGCCAAACACTTCTC |
| Internal bar code: | GGAGAGTTGGTGGCTGCTAACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 485 |
| LEAP-Seq percent confirming: | 97.9154 |
| LEAP-Seq n confirming: | 7891 |
| LEAP-Seq n nonconfirming: | 168 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGTGTCCACAGACGAACC |
| Suggested primer 2: | GTACCTGACGGAGGTTCTCG |