Insertion junction: LMJ.RY0402.132300_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.132300
Chromosome:chromosome 14
Location:2799799
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g627350 XYI3 Sulfite exporter family protein; (1 of 4) PTHR14255:SF3 - SULFITE EXPORTER TAUE/SAFE FAMILY PROTEIN intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACACACAACCAAACACACACACACAATC
Internal bar code:CACAGTCCCGGGATGTGGCCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:341
LEAP-Seq percent confirming:1.28405
LEAP-Seq n confirming:454
LEAP-Seq n nonconfirming:34903
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGTCGTGATGTGGATCAG
Suggested primer 2:GCTTTTGGGGCTGTGTGTAT