| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.132326 |
| Chromosome: | chromosome 16 |
| Location: | 6804931 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAATATGCACGGAATGCTGGACTGTGCTAG |
| Internal bar code: | GTTGGCTAATTCGCGTATACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1155 |
| LEAP-Seq percent confirming: | 99.4222 |
| LEAP-Seq n confirming: | 14109 |
| LEAP-Seq n nonconfirming: | 82 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTACGCAGCCTCGTAAGGT |
| Suggested primer 2: | GATAAGGGTTGCGCATGAGT |