Insertion junction: LMJ.RY0402.132333_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.132333
Chromosome:chromosome_7
Location:5180833
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre07.g348450 sense 5'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):CTCTCGACAAACATTTCAGTAAGACACTCA
Internal bar code:GCCCGAGTGTGAACCGAAGCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:829
LEAP-Seq percent confirming:99.5345
LEAP-Seq n confirming:2138
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CCGAACAGTCGGAAGCTAAG
Suggested primer 2:GTAGGCAATTCGGAACGTGT