Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.132333 |
Chromosome: | chromosome_7 |
Location: | 5180833 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre07.g348450 | sense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTCTCGACAAACATTTCAGTAAGACACTCA |
Internal bar code: | GCCCGAGTGTGAACCGAAGCAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 829 |
LEAP-Seq percent confirming: | 99.5345 |
LEAP-Seq n confirming: | 2138 |
LEAP-Seq n nonconfirming: | 10 |
LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCGAACAGTCGGAAGCTAAG |
Suggested primer 2: | GTAGGCAATTCGGAACGTGT |