| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.132333 |
| Chromosome: | chromosome 10 |
| Location: | 3362722 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g444000 | (1 of 2) IPR007743//IPR027417//IPR030385 - Immunity-related GTPases-like // P-loop containing nucleoside triphosphate hydrolase // IRG-type guanine nucleotide-binding (G) domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTGGTAGGGTCGCCCCAATCGCTATTCC |
| Internal bar code: | CGGTCGCCTTCGCGTATACTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 342 |
| LEAP-Seq percent confirming: | 99.1288 |
| LEAP-Seq n confirming: | 4096 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCCCGTTAGTGAATTGCT |
| Suggested primer 2: | CTGCTGTGCATCGGAGACTA |