| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.132365 |
| Chromosome: | chromosome 13 |
| Location: | 3482180 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g587700 | SDR33 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTAGTGGCAGTGGTCGGGATATGTGCGGT |
| Internal bar code: | TGGCCCCAGCTACAGGGCCCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 369 |
| LEAP-Seq percent confirming: | 91.379 |
| LEAP-Seq n confirming: | 5427 |
| LEAP-Seq n nonconfirming: | 512 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACCCAATGCAGTAGACCG |
| Suggested primer 2: | TTTGGGTTTAGTTTGGGCTG |