| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.132372 |
| Chromosome: | chromosome 13 |
| Location: | 1484635 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g572550 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCATACTGTATTTTGGGGCCATGCTTGCG |
| Internal bar code: | GAGGCCCTGAATCACATCGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 688 |
| LEAP-Seq percent confirming: | 99.3509 |
| LEAP-Seq n confirming: | 5510 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCTGTACCATCGACAACG |
| Suggested primer 2: | GATGTTGGCTGCTAGGCTTC |