| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.132387 |
| Chromosome: | chromosome 6 |
| Location: | 8678403 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g309650 | (1 of 1) IPR003200//IPR006626//IPR011050 - Nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase-like // Parallel beta-helix repeat // Pectin lyase fold/virulence factor | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTTCCCCACGCGGCCGCGCCTCTCGCC |
| Internal bar code: | AAGGGCCAGAGAATACAGGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 752 |
| LEAP-Seq percent confirming: | 99.9742 |
| LEAP-Seq n confirming: | 3871 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTGCGTGTTTTGTGTTGG |
| Suggested primer 2: | CAGATAGTCGCCAAGCACAA |