| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.132404 |
| Chromosome: | chromosome 4 |
| Location: | 2004732 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g218850 | RNP | (1 of 1) K14846 - ribosome production factor 1 (RPF1); Putative small nucleolar ribonucleoprotein U3 protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAAGCTGAGAGCTGCTGTGTTGGAGCCA |
| Internal bar code: | GGCCCATGATCCGTAACGAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 724 |
| LEAP-Seq percent confirming: | 99.7754 |
| LEAP-Seq n confirming: | 7108 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGAAACCTCATGCAGCAA |
| Suggested primer 2: | GTTCTCATCCGCCATGATCT |