| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.132407 |
| Chromosome: | chromosome 2 |
| Location: | 4571962 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g100900 | (1 of 102) PF01753 - MYND finger (zf-MYND) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCTTGCCCAGCGTCGCAATCAGCGATGC |
| Internal bar code: | TAAGGGGCCGTGCCGGGAGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 148 |
| LEAP-Seq percent confirming: | 95.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTGCTTGGAACCTGTG |
| Suggested primer 2: | CTTCCAGGTAGCTCACCCAC |