| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.132417 |
| Chromosome: | chromosome_1 |
| Location: | 3544377 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g022750 | FAP159 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGTTTCTTGTGTGTGCGTACCGGTACTTGA |
| Internal bar code: | TATGGCACATATTCTCGATTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1153 |
| LEAP-Seq percent confirming: | 99.113 |
| LEAP-Seq n confirming: | 8269 |
| LEAP-Seq n nonconfirming: | 74 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATGGCCAACTCTACCATT |
| Suggested primer 2: | CTACGAGGCACGAAGATGTG |