Insertion junction: LMJ.RY0402.132424_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.132424
Chromosome:chromosome 2
Location:2464446
Confidence (%):95
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGGAGTATGCTTGGATGACCAGTAGGTC
Internal bar code:TAGTTCCAGGTGTGACTCCCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:777
LEAP-Seq percent confirming:28.5714
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:30
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GCAAAGAAGGAGCACCAGTC
Suggested primer 2:CTAGACCAGACGGCTTGAGG