| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.132429 |
| Chromosome: | chromosome 1 |
| Location: | 3050759 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g018750 | DIV155 | (1 of 44) IPR000048 - IQ motif, EF-hand binding site | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTACCCCATGCAGCCGTACGGCAGCAGG |
| Internal bar code: | GAACGTATCGGCGCCGGAGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 884 |
| LEAP-Seq percent confirming: | 99.9079 |
| LEAP-Seq n confirming: | 14101 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTGCTTACGGTGGTTGTG |
| Suggested primer 2: | TTGAGGTGTAGATCGAGGGG |