Insertion junction: LMJ.RY0402.132448_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.132448
Chromosome:chromosome 7
Location:2933880
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g332750 PSL2 Protein of unknown function; (1 of 19) IPR000225//IPR016024 - Armadillo // Armadillo-type fold CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCGGGCCAGCCGCAGGTCGGGGTGGGCG
Internal bar code:TTTTAGGGGACACAACAGATTA

Confirmation - LEAP-Seq

LEAP-Seq distance:306
LEAP-Seq percent confirming:53.719
LEAP-Seq n confirming:130
LEAP-Seq n nonconfirming:112
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:ACAACTTCATCCTCCCCTCC
Suggested primer 2:GAGATGTTCTGCGAGGGAAG