| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.132461 |
| Chromosome: | chromosome 8 |
| Location: | 3955976 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g378700 | (1 of 2) K13173 - arginine and glutamate-rich protein 1 (ARGLU1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAAACGTGCGCGCGTCTGTTACTCGCGAA |
| Internal bar code: | ATGGGATCAGACGTGTGAACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 409 |
| LEAP-Seq percent confirming: | 99.6215 |
| LEAP-Seq n confirming: | 6316 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCTTAGCGTTTGCCCTGA |
| Suggested primer 2: | TGGCTAGTTTGGATGCACAG |