| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.132464 |
| Chromosome: | chromosome 8 |
| Location: | 4236818 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g380600 | (1 of 1) K08287 - dual-specificity kinase (E2.7.12.1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTTAAAACGAAACGTCCTTTGCGTGTCA |
| Internal bar code: | TCACGAGCGTGGGGGGAAACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 846 |
| LEAP-Seq percent confirming: | 99.6345 |
| LEAP-Seq n confirming: | 1363 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCCTGTAGTCGTTCTGGC |
| Suggested primer 2: | ACCTGGCAACGACATACCTC |