| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.132491 |
| Chromosome: | chromosome 2 |
| Location: | 5594444 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g109050 | OPR5 | OctotricoPeptide Repeat protein 5 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCAGCAACGGAGAGCCGCACCTATCCGG |
| Internal bar code: | CGATAAATCTGCCTTCAGGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 694 |
| LEAP-Seq percent confirming: | 98.4791 |
| LEAP-Seq n confirming: | 7252 |
| LEAP-Seq n nonconfirming: | 112 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTGAGTCCTAGCCAAGCC |
| Suggested primer 2: | CTAGCCGAGGACAGGAACAG |