Insertion junction: LMJ.RY0402.132500_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.132500
Chromosome:chromosome 10
Location:19391
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g417550 CLPS3,CLPT3 (1 of 2) 3.6.4.10 - Non-chaperonin molecular chaperone ATPase / Molecular chaperone Hsc70 ATPase; Additional subunit of chloroplast ClpP complex intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGGTGGAGGATTCGCATACAGGCTCAGCT
Internal bar code:TCTTCATATCTTCCGAAGAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:824
LEAP-Seq percent confirming:99.6941
LEAP-Seq n confirming:9778
LEAP-Seq n nonconfirming:30
LEAP-Seq n unique pos:42

Suggested primers for confirmation by PCR

Suggested primer 1:TCCTAACCGCTGCTATTGCT
Suggested primer 2:CTGGTGAGGTATGTGGGCTT