| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.132541 |
| Chromosome: | chromosome 16 |
| Location: | 3166587 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g666050 | CPLD49,SCD1 | (1 of 4) PF03435 - Saccharopine dehydrogenase NADP binding domain (Sacchrp_dh_NADP); Cytochrome b6f biogenesis protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGATGCCCCTGCTTGCAGCAGCACTCTCA |
| Internal bar code: | CTGGCGACTACCGTGGCCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 695 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCACCTCCTTGTTGTCCT |
| Suggested primer 2: | CTCGCAGAATTGGGACTAGC |