| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.132549 |
| Chromosome: | chromosome 13 |
| Location: | 1159877 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g569651 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCAAACACGATGGGGTGGACCCAAATGC |
| Internal bar code: | ACGCCGGGCGGGAAATTTCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 873 |
| LEAP-Seq percent confirming: | 98.7057 |
| LEAP-Seq n confirming: | 8694 |
| LEAP-Seq n nonconfirming: | 114 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGTTCATCTGGGTTTGAA |
| Suggested primer 2: | GCTGCCCATTATTCAAGCAT |