| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.132551 |
| Chromosome: | chromosome 16 |
| Location: | 6060696 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676200 | (1 of 3) PTHR10751:SF2 - GUANYLATE-BINDING PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGACGAGCGACGGTAGCGAAAGAAATTAA |
| Internal bar code: | AGGCTCTTGATTATGCAGTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 128 |
| LEAP-Seq percent confirming: | 67.9091 |
| LEAP-Seq n confirming: | 1166 |
| LEAP-Seq n nonconfirming: | 551 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTATGCTGCCAACCCAGTT |
| Suggested primer 2: | CGGTTTGGGAAACAGCTTTA |