| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.132571 |
| Chromosome: | chromosome 2 |
| Location: | 374203 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g075850 | STK12,SNRK2A,SNRK2,STPK12,ARS11 | (1 of 107) IPR000719//IPR002290//IPR011009//IPR020635 - Protein kinase domain // Serine/threonine/dual specificity protein kinase, catalytic domain // Protein kinase-like domain // Tyrosine-protein kinase, catalytic domain; snRK1 family in Chlamydomonas, subgroup 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGGCTGGACGGTGTCGGAGGCCGTGGAG |
| Internal bar code: | ATCATTGCCGCAGGCTGGAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1203 |
| LEAP-Seq percent confirming: | 99.0625 |
| LEAP-Seq n confirming: | 634 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATGAGTCATTGCTAGCCG |
| Suggested primer 2: | GTGAGTGCAAAGGGGTCAAT |