| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.132571 |
| Chromosome: | chromosome 13 |
| Location: | 3567431 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g588201 | RAP6,OPR58 | OctotricoPeptide Repeat protein 58; (1 of 19) PTHR21228//PTHR21228:SF20 - FAST LEU-RICH DOMAIN-CONTAINING // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGACCGGAGGAGCGGCGGCGGTGACTGGA |
| Internal bar code: | TCTACAGGTCGATCCGACTCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 87 |
| LEAP-Seq percent confirming: | 93.2967 |
| LEAP-Seq n confirming: | 849 |
| LEAP-Seq n nonconfirming: | 61 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGATATAGGCACCAGCAGA |
| Suggested primer 2: | TGTGTGTGTGTGTGAGGTGG |