| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.132572 |
| Chromosome: | chromosome 2 |
| Location: | 6148639 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g113500 | (1 of 1) IPR001322//IPR014867 - Lamin Tail Domain // Spore coat protein CotH | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGGCTGCTGTCGTTGCCGTCCTCCTCGT |
| Internal bar code: | TGCTCGGTGCGCGTATTGATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 326 |
| LEAP-Seq percent confirming: | 93.5115 |
| LEAP-Seq n confirming: | 10535 |
| LEAP-Seq n nonconfirming: | 731 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACGCTGTGGGGTTATCAT |
| Suggested primer 2: | TCTGACCTGTCACTTGCAGC |