| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.132600 |
| Chromosome: | chromosome 14 |
| Location: | 1696550 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619400 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGGCAAGGAAGGGCGGCAAGTACGGCA |
| Internal bar code: | GTGGCGTCGGAAGCACTTATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 116 |
| LEAP-Seq percent confirming: | 97.9541 |
| LEAP-Seq n confirming: | 1963 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTCGATTTCGTACCTGGA |
| Suggested primer 2: | GGGAGCTGTACCTGCTCAAG |