| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.132627 |
| Chromosome: | chromosome 9 |
| Location: | 4032824 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393099 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCTGTGAACTTTTTCCTTCCGACAGGCA |
| Internal bar code: | CTTATAGCTTGTCTGGGGTAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 369 |
| LEAP-Seq percent confirming: | 99.4826 |
| LEAP-Seq n confirming: | 2115 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTACCGAGCATGATAAGCA |
| Suggested primer 2: | CGCAACCTGTGTCTGTGTCT |