Insertion junction: LMJ.RY0402.132627_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.132627
Chromosome:chromosome 9
Location:5481218
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g400293 (1 of 10) IPR029068 - Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGCAATGCCCACCGCGGTGCCGGGCAGG
Internal bar code:GATCATCACGTCAAACGATCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.0
LEAP-Seq n confirming:0
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:0

Suggested primers for confirmation by PCR

Suggested primer 1:AATCACCCAGCTCTTCCCTT
Suggested primer 2:ACTAAAGCGCGAGAGCTGAC