| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.132627 |
| Chromosome: | chromosome 9 |
| Location: | 5481225 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400293 | (1 of 10) IPR029068 - Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGTGAGGCGGGAGGCATGAGGATGGGAT |
| Internal bar code: | AGACGGAAGGAGCTCGGGTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 292 |
| LEAP-Seq percent confirming: | 62.7119 |
| LEAP-Seq n confirming: | 222 |
| LEAP-Seq n nonconfirming: | 132 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATCACCCAGCTCTTCCCTT |
| Suggested primer 2: | ACTAAAGCGCGAGAGCTGAC |