| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.132652 |
| Chromosome: | chromosome 1 |
| Location: | 7541575 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g054300 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGGCAATCCGGCCTGATGTGCATTGAGA |
| Internal bar code: | GAACAATAGACAACTGCGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 749 |
| LEAP-Seq percent confirming: | 99.7678 |
| LEAP-Seq n confirming: | 8595 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCAAGAGTGAGTGGCTTTC |
| Suggested primer 2: | CGGTTGACCAATGCATACAG |